Graduation Date

Spring 2026

Document Type

Thesis

Program

Master of Science degree with a major in Biology

Committee Chair Name

Oscar Vargas

Committee Chair Affiliation

Cal Poly Humboldt Faculty or Staff

Second Committee Member Name

Erik Jules

Second Committee Member Affiliation

Cal Poly Humboldt Faculty or Staff

Third Committee Member Name

Rachael Wade

Third Committee Member Affiliation

Cal Poly Humboldt Faculty or Staff

Fourth Committee Member Name

Ho Yi Wan

Fourth Committee Member Affiliation

Cal Poly Humboldt Faculty or Staff

Sixth Committee Member Affiliation

Community Member or Outside Professional

Keywords

Erysimum, Erysimum menziesii, Erysimum concinnum, Evolution, Systematics, California Floristic Province, Microevolution, Budding speciation, Convergent evolution, Genetics, Phylogenetics, Ecological speciation, Cryptic species, Dunes

Abstract

Morphological similarity among geographically isolated populations can obscure deep evolutionary divergence, especially when convergent evolution under similar environmental conditions yields comparable phenotypes across independent lineages. Such cryptic diversity is likely underestimated in hotspots like the California Floristic Province (CFP), a global biodiversity hotspot, defined by its Mediterranean-like climate and encompassing most of California, southwestern Oregon, and northwest Baja California. The CFP is home to a disproportionate number of narrow endemics whose origins and evolutionary relationships remain poorly understood. Here, I investigate the evolutionary origins of Erysimum menziesii (Brassicaceae), a federally endangered California coastal dune endemic whose fragmented distribution has led to its assumed paleoendemic status, despite limited phylogenetic investigation. Its close morphological similarity and overlapping distribution with Erysimum concinnum have blurred the species boundaries between the two taxa, with both hypothesized to be derived from Erysimum capitatum. Using ddRADseq across 182 individuals spanning 31 populations from Del Norte County to Monterey County, I assessed the phylogenetic position of E. menziesii relative to E. concinnum and E. capitatum, evaluated the speciation mechanism, and assessed genetic differentiation within and among species using STRUCTURE, principal component analysis, and pairwise Fst. I found that both E. menziesii and E. concinnum form a cryptic species complex, with each species comprising four independent lineages that have repeatedly colonized similar coastal environments, with E. menziesii occupying coastal dunes and E. concinnum occupying coastal bluffs. High pairwise differentiation among E. menziesii lineages (Fst = 0.205-0.602), combined with their morphological similarity, reflects convergent evolution driven by similar selective pressure found in dune system, supporting multiple independent neoendemic origins. In Mendocino and Monterey Counties, phylogenetic and genetic evidence support budding speciation from proximal coastal progenitors rather than the suspected E. capitatum. Current species boundaries in both taxa fail to reflect the underlying evolutionary diversity, with each lineage warranting recognition as a distinct taxonomic unit and independent management for conservation.

Citation Style

Chicago-style

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