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Publication Date
2020
Abstract
ASR is a cyanobacterial light-detecting transmembrane protein, which communicates to the cell through its soluble transducer ASRT. In this study, we describe a method for cloning ASRT using PCR, vector construction, and transformation for protein expression. We have made progress in cloning and continuing work on purification protocols. We will use immobilized metal affinity chromatography to purify ASRT, and use this protein in subsequent studies on its interaction with ASR. Characterization of ASR’s signal transduction through ASRT has revealed thus far a striking similarity to eukaryotic GPCRs and has potential for use in more cost-effective and precise expression induction in bacterial systems.
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