Graduation Date

Fall 2025

Document Type

Thesis

Program

Master of Science degree with a major in Biology

Committee Chair Name

Allison Bronson

Committee Chair Affiliation

Cal Poly Humboldt Faculty or Staff

Second Committee Member Name

John Reiss

Second Committee Member Affiliation

Cal Poly Humboldt Faculty or Staff

Third Committee Member Name

Darren Ward

Third Committee Member Affiliation

Cal Poly Humboldt Faculty or Staff

Fourth Committee Member Name

Melanie Stiassny

Fourth Committee Member Affiliation

Community Member or Outside Professional

Keywords

Olfaction, Morphology, Rod cells, Olfactory rosette

Subject Categories

Biology

Abstract

Rainbow trout (Oncorhynchus mykiss) are an ecologically and culturally important salmonid species that exhibits diverse life history strategies, including anadromous and resident freshwater life cycles. Though many studies have examined their olfactory morphology, none have directly compared possible olfactory microstructural differences between wild and hatchery-raised populations. Histology and scanning electron microscopy (SEM) were employed to compare the gross anatomy and ultrastructure of olfactory rosettes in three O. mykiss populations: wild residents from Jacoby Creek (JC), a resident lineage of hatchery fish from Mad River Hatchery (MRH), and an anadromous lineage from the Cal Poly Humboldt (CPH) Hatchery. The CPH population was also assessed after exposure to an anesthetic dose of buffered tricaine methanesulfonate (MS-222) following observations in 2022 of abnormal olfactory epithelia and rod cells. No morphological differences were observed between anesthetized and control CPH fish. All populations possessed olfactory receptor cells within the sensory epithelium. However, olfactory sensory regions between secondary lamellae were dominated by rod cells in CPH Hatchery fish, sporadically present in JC wild residents but absent in MRH residents. While rod cells have been documented in numerous fish species, their function remains unknown. Though olfaction is key in salmonid homing, foraging, and conspecific communication, without knowing the functional significance of rod cells and differences in rosette maturation, it is unclear whether their presence may impact the behavior or survival of hatchery trout.

Citation Style

APA

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