Publication Date

2023

College

College of Natural Resources & Sciences

Department

Chemistry

Document Type

Article

Description

The CB2 G-protein coupled receptors (GPCR) is found in the brainstem & hippocampus and is devoid of psychotropic effects but is less studied than the CB1 receptor. CB2 is inducible in CNS microglia following inflammation or injury, indicating a role in pain response. Here we sought to analyze CB2 using ChimeraX structures and overcome GPCR protein insolubility in extraction. The pET28a-CNR2, plasmid created and transformed into E.coli pLysS, was confirmed by restriction digest. Purification of CB2 micelles was achieved by affinity chromatography with detergent (43 kD). We aim to utilize nanodiscs to stabilize CB2, allowing studies of the molecular underpinnings informing treatment options.

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