Comparative Analysis of Anabaena Sensory Rhodopsin in Nanodiscs Containing Various Lipids.
College of Natural Resources & Sciences
Membrane proteins (MP) are crucial for cell pathways, but are difficult to study due to hydrophobicity. Nanodiscs (ND) provide a lipid bilayer mimetic enabling MP studies. To improve efficiency, we compared two ND assembly methods for detergent removal, and evaluated how lipid affects the MP, Anabaena Sensory Rhodopsin. ND were assembled with purified ASR, various lipids, Sodium Cholate and Apolipoprotein AI. Electrophoresis was utilized to evaluate complex formation. ASR was successfully isolated and assembled into ND. We found that the biobead method was faster and had less risk of loss, showing it to be the better method. ND provide a promising approach to therapeutics affecting MP.