Graduation Date

Spring 2023

Document Type

Thesis

Program

Master of Arts degree with a major in Psychology, option Academic Research

Committee Chair Name

Dr. Ethan Gahtan

Committee Chair Affiliation

HSU Faculty or Staff

Second Committee Member Name

Dr. Amanda Hahn

Second Committee Member Affiliation

HSU Faculty or Staff

Third Committee Member Name

Dr. Amber Gaffney

Third Committee Member Affiliation

HSU Faculty or Staff

Keywords

Area postrema, MS-222, Zebrafish, Anesthesia, Sedation, Voltage-gated sodium channel, Circumventricular organ, Calcium imaging, GCaMP

Subject Categories

Psychology

Abstract

MS-222 is a common fish anesthetic that dampens neural activity by inhibiting neuronal voltage-gated sodium channels (Nav) required for action potentials. Previous experiments conducted by our lab’s primary investigator using fluorescence calcium imaging in zebrafish neurons resulted in the observation that neurons in the area postrema (AP), a circumventricular organ located in the dorsal hindbrain, displayed prominent fluorescence intensity during MS-222 induced sedation. I used the genetically encoded calcium indicator GCaMP6s to conduct fluorescence calcium imaging with the goal of recording neural activity in the larval zebrafish area postrema and optic tectum to compare the anesthetic effects of MS-222 across brain regions. A 2 x 2 analysis of variance (ANOVA) was used to calculate the differences in fluorescence intensity before and after MS-222 in both brain regions. Contrary to our hypothesis, the results of the experiment indicate that MS-222 had an insignificant effect on the change in fluorescence intensity in each brain region and although insignificant, appears to slightly increase area postrema and optic tectum mean standard deviations. These results are theoretically inconsistent with the literary understanding of how voltage-gated sodium channel antagonism affects cellular behavior. A small sample size with high variance is the most likely explanation for these results.

Citation Style

APA

Share

 
COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.