The effect of the loss of Lgl1 in murine neural progenitor cells on MAPK signaling and proliferation
Graduation Date
Fall 2018
Document Type
Thesis
Program
Master of Science degree with a major in Biology
Committee Chair Name
Dr. Amy Sprowles
Committee Chair Affiliation
HSU Faculty or Staff
Second Committee Member Name
Dr. Jenny Cappuccio
Second Committee Member Affiliation
HSU Faculty or Staff
Third Committee Member Name
Dr. Jacob Varkey
Third Committee Member Affiliation
HSU Faculty or Staff
Fourth Committee Member Name
Dr. Bruce O'Gara
Fourth Committee Member Affiliation
HSU Faculty or Staff
Subject Categories
Biology
Abstract
Glioblastoma is an incurable, aggressive, and highly invasive type of brain tumor that harbors tumor initiating cells characterized by disrupted polarized cell divisions. A cell polarity gene lethal (2) giant larvae 1 (Lgl1) has been implicated in gliomas and is a tumor suppressor initially identified in Drosophila with roles in proliferation. The loss of Lgl1 in Drosophila activates the MAPK protein kinase JNK and the Ras pathway and therefore its downstream kinase ERK, a transcription factor modulator. Furthermore, when Lgl1 is knocked out in mice, a phenotype similar to glioma is seen. Loss of the human form of Lgl1, Hugl1, and increases in c-Jun, an oncogene and JNK target, has been associated with glioma in humans. Additionally, the protooncogene transcription factor c-Myc is documented in glioma to directly correlate to tumor grade and an increase in an analogous form, d-Myc, in Drosophila has been shown to promote survival of Lgl mutants through a Ras mechanism. Here we sought to determine if the cancer properties associated with loss of Lgl in mice and humans are related to changes in MAPK signaling. To accomplish this, murine neural progenitor cells from the subventricular zone of mice with a Lgl1 knockout were cultured in vitro. These cells were plated adherently and characterized for changes in phosphorylation states of MAPK proteins ERK, JNK and p38 as well as two protooncogene MAPK downstream targets, c-Jun and c-Myc. In addition, to understand if MAPK phosphorylation is related to proliferation we characterized the proliferation rates of these cells in the presence of chemical inhibitors of p38 and ERK’s upstream activating kinase MEK. Differential expression patterns were observed in MAPK proteins and their downstream targets associated with the loss of Lgl1, under standard conditions and with the treatment of DMSO as a drug vehicle control and chemical inhibitors of p38 and MEK. Additionally, it was found that the loss of Lgl1 in neurosphere culture slightly increased growth and under adherent conditions this effect was not seen, however, changes did occur in the presence of p38 and MEK inhibitors. This supports previous data and signifies the importance of MAPK pathway in cancer phenotypes and beginning to characterize the role of the Lgl1 protein in the mouse.
Citation Style
Cell Journal
Recommended Citation
LaCourse, Monique R., "The effect of the loss of Lgl1 in murine neural progenitor cells on MAPK signaling and proliferation" (2018). Cal Poly Humboldt theses and projects. 236.
https://digitalcommons.humboldt.edu/etd/236