College of Natural Resources & Sciences
Utilizing cellulase enzymes can enhance the production of biofuels. In this study, 14 cellulases identified through metagenomic analysis of cow rumen were expressed in E. Coli, purified using immobilized metal affinity chromatography (IMAC), and then assessed for enzymatic activity versus a control cellulase isolated from Aspergillus Niger. To do this, we evaluated the breakdown of carboxymethylcellulose (CMC) in a plate assay with Congo Red detection. 4 with high activity and 2 with low activity were selected for analyzing the effective pH on the enzymatic activity and expanding the research to kinetic analysis. Our results could inform new cellulase design and enhance biofuel production.