Identification of two uncharacterized proteins that physically interact with the Caenorhabditis elegans SPE-26 protein

Graduation Date

2001

Document Type

Thesis

Program

Other

Program

Thesis (M.A.)--Humboldt State University, Biology, 2001

Committee Chair Name

Jacob Varkey

Committee Chair Affiliation

HSU Faculty or Staff

Keywords

Nematodes--Physiology, Caenorhabditis elegans, Humboldt State University -- Theses -- Biology

Abstract

The Caenorhabditis elegans SPE-26 protein is involved in the formation of viable spermatids. Two-hybrid interaction analysis was performed in order to determine with which proteins SPE-26 physically interacts. Fusing the carboxy terminal 471 amino acids of the SPE-26 protein to the Escherichia coli LexA protein created a bait protein. This bait was then used to screen a C. elegans cDNA library for potential protein-protein interactions. This library consisted of C. elegans cDNA clones fused to the transcription activator, B42, an E. coli gene that produces a protein that has transcription activating activity. Potential physical interactions were uncovered with two different uncharacterized C. elegans proteins, the collagen-like protein F26B1.4 and the DNAJ-like protein F39B2.10. DNAJ proteins from mice, humans, and rats have been found to be expressed in germ cells, while some collagen proteins are believed to be involved in C. elegans fertility.

https://scholarworks.calstate.edu/concern/theses/gb19f7979

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